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AQP1 Rabbit mAb#49447

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Product Detail

Product NameAQP1 Rabbit mAb

Clone No.JM10-98

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAQP 1 antibody
AQP CHIP antibody
AQP-1 antibody
AQP1 antibody
AQP1_HUMAN antibody
aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
aquaporin 1 (Colton blood group) antibody
Aquaporin CHIP antibody
Aquaporin-1 antibody
Aquaporin-CHIP antibody
Aquaporin1 antibody
Channel forming integral protein 28kDa antibody
Channel like integral membrane protein 28 kDa antibody
CHIP 28 antibody
CHIP28 antibody
CO antibody
Colton blood group antibody
Growth factor induced delayed early response protein antibody
MGC26324 antibody
Urine water channel antibody
Water channel protein CHIP 29 antibody
Water channel protein CHIP29 antibody
Water channel protein for red blood cells and kidney proximal tubule antibody

Accession NoSwiss-Prot#:P29972

Uniprot P29972

Gene ID 358;

Calculated MW28/35 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of AQP1 on different cells lysates using anti-AQP1 antibody at 1/500 dilution. Positive control:
Line 1: Hela
Line 2:Jurkat
Line 3: Human kidney
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-AQP1 antibody. Counter stained with hematoxylin.
ICC staining AQP1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AQP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AQP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of Aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most Aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP1 is an integral membrane protein expressed in erythrocytes and renal tubule cells.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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