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VAMP2 Rabbit mAb#49463

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Product Detail

Product NameVAMP2 Rabbit mAb

Clone No.JM11-00

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IP, ICC/IF, FC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic peptide within Human VAMP2 aa 9-42 / 116.

ConjugateUnconjugated

Other NamesFLJ11460 antibody
RATVAMPB antibody
RATVAMPIR antibody
SYB antibody
SYB2 antibody
Synaptobrevin 2 antibody
Synaptobrevin-2 antibody
VAMP 2 antibody
VAMP-2 antibody
Vamp2 antibody
VAMP2_HUMAN antibody
Vesicle associated membrane protein 2 antibody
Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
Vesicle-associated membrane protein 2 antibody

Accession NoSwiss-Prot#:P63027

Uniprot P63027

Gene ID 6844;

Calculated MW15 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:500-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY5Y Lane 2: Jurkat
ICC staining VAMP2 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VAMP2 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VAMP2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY5Y cells with VAMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and γ-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding.

If you have published an article using product 49463, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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