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ATP5A1 Rabbit mAb#49466

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Product Detail

Product NameATP5A1 Rabbit mAb

Clone No.JM110-04

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen DescA synthesized peptide derived from human ATP5A

ConjugateUnconjugated

Other NamesATP synthase alpha chain, mitochondrial antibody
ATP synthase subunit alpha antibody
ATP synthase subunit alpha mitochondrial antibody
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
ATP sythase (F1 ATPase) alpha subunit antibody
ATP5A antibody
Atp5a1 antibody
ATP5AL2 antibody
ATPA_HUMAN antibody
ATPM antibody
Epididymis secretory sperm binding protein Li 123m antibody
hATP1 antibody
HEL-S-123m antibody
MC5DN4 antibody
mitochondrial antibody
Mitochondrial ATP synthetase antibody
Mitochondrial ATP synthetase oligomycin resistant antibody
Modifier of Min 2 mouse homolog antibody
Modifier of Min 2, mouse, homolog of antibody
MOM2 antibody
OMR antibody
ORM antibody
OTTHUMP00000163475 antibody

Accession NoSwiss-Prot#:P25705

Uniprot P25705

Gene ID 498;

Calculated MW50 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200
FC 1:20-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
ICC staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATP5A1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATP5A1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Western blot analysis of ATP5A1 expression in HepG2 cell lysate

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one e chain. F0 consists of three subunits: a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.

If you have published an article using product 49466, please notify us so that we can cite your literature.

et al,Multifunctional manganese-based nanogels catalyze immune energy metabolism to promote bone repair , (2025),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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