Product Detail
Product NameCaMKII alpha Rabbit mAb
Clone No.JM11-07
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, IP, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesAlpha CaMKII antibody
Calcium calmodulin dependent protein kinase II antibody
Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
Calcium/calmodulin dependent protein kinase type II alpha chain antibody
Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
Calcium/calmodulin-dependent protein kinase II alpha antibody
Calcium/calmodulin-dependent protein kinase II-alpha antibody
Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
Calcium/calmodulin-dependent protein kinase type IIA antibody
CaM kinase II alpha chain antibody
CaM kinase II alpha subunit antibody
CaM kinase II subunit alpha antibody
CaMK II alpha subunit antibody
CaMK-II subunit alpha antibody
Camk2a antibody
CAMKA antibody
CaMKII antibody
CaMKIINalpha antibody
EC 2.7.11.17 antibody
KCC2A_HUMAN antibody
KIAA0968 antibody
MGC123320 antibody
MGC139375 antibody
MGC155201 antibody
mKIAA0968 antibody
PK2CDD antibody
PKCCD antibody
R74975 antibody
zgc:112538 antibody
zgc:123320 antibody
Accession NoSwiss-Prot#:Q9UQM7
Uniprot
Q9UQM7
Gene ID
815;
Calculated MW54 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.
Flow cytometric analysis of SH-SY5Y cells with CaMKII alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.
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