Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Recombinant Rabbit Monoclonal Antibodies > VCP Rabbit mAb

VCP Rabbit mAb#49469

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameVCP Rabbit mAb

Clone No.JM11-15

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC, IP

Species ReactivityHu, Ms, Rt, Zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names15S Mg(2+) ATPase p97 subunit antibody
15S Mg(2+)-ATPase p97 subunit antibody
ALS14 antibody
ATPase p97 antibody
CDC48 antibody
IBMPFD antibody
MGC131997 antibody
MGC148092 antibody
MGC8560 antibody
p97 antibody
TER ATPase antibody
TERA antibody
TERA_HUMAN antibody
Transitional endoplasmic reticulum ATPase antibody
Valosin containing protein antibody
Valosin-containing protein antibody
VCP antibody
Yeast Cdc48p homolog antibody

Accession NoSwiss-Prot#:P55072

Uniprot P55072

Gene ID 7415;

Calculated MW89 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution. Positive control�� Line 1: SH-SY5Y Line 2: HepG2 Line 3: Hela
Western blot analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin.
ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VCP in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HL-60 cells with VCP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.

If you have published an article using product 49469, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors