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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PABPN1 Rabbit mAb

PABPN1 Rabbit mAb#49479

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Product Detail

Product NamePABPN1 Rabbit mAb

Clone No.JM11-28

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC, IP

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesNuclear poly(A)-binding protein 1 antibody
OPMD antibody
PAB2 antibody
PABII antibody
PABP 2 antibody
pABP-2 antibody
PABP2 antibody
PABP2_HUMAN antibody
PABPII antibody
Pabpn1 antibody
poly(A) binding protein nuclear 1 antibody
Poly(A)-binding protein 2 antibody
Poly(A)-binding protein II antibody
PolyA binding protein II antibody
Polyadenylate-binding nuclear protein 1 antibody
Polyadenylate-binding protein 2 antibody

Accession NoSwiss-Prot#:Q86U42

Uniprot Q86U42

Gene ID 8106;

Calculated MW49 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of PABPN1 on MCF-7 cells lysates using anti-PABPN1 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PABPN1 antibody. Counter stained with hematoxylin.
ICC staining PABPN1 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.


ICC staining PABPN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.


Flow cytometric analysis of Hela cells with PABPN1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.

If you have published an article using product 49479, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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