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MUC2 Rabbit mAb#49518

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Product Detail

Product NameMUC2 Rabbit mAb

Clone No.JA10-92

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesIntestinal mucin 2 antibody
Intestinal mucin-2 antibody
MLP antibody
MUC 2 antibody
MUC-2 antibody
Muc2 antibody
MUC2_HUMAN antibody
Mucin 2 antibody
Mucin 2 intestinal antibody
Mucin 2 intestinal/tracheal antibody
Mucin 2 oligomeric mucus/gel forming antibody
Mucin 2 precursor antibody
Mucin 2 tracheal antibody
Mucin like protein antibody
Mucin-2 antibody
Mucin2 antibody
SMUC antibody

Accession NoSwiss-Prot#:Q02817

Uniprot Q02817

Calculated MW540 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.
ICC staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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