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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PKA 2 beta (regulatory subunit) Rabbit mAb

PKA 2 beta (regulatory subunit) Rabbit mAb#49527

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Product Detail

Product NamePKA 2 beta (regulatory subunit) Rabbit mAb

Clone No.JA11-69

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IP, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAI451071 antibody
AW061005 antibody
cAMP dependent protein kinase type II beta regulatory chain antibody
cAMP dependent protein kinase type II beta regulatory subunit antibody
cAMP-dependent protein kinase type II-beta regulatory subunit antibody
H RG363E19.2 antibody
KAP3_HUMAN antibody
MGC116401 antibody
Pkarb2 antibody
PRKAR 2 antibody
PRKAR2 antibody
PRKAR2B antibody
Protein kinase cAMP dependent regulatory type II beta antibody
RATDNA antibody
RII beta antibody
RII(beta) antibody
RIIbeta antibody
WUGSC:H RG363E19.2 antibody

Accession NoSwiss-Prot#:P31323

Uniprot P31323

Gene ID 5577;

Calculated MW46 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- PKA 2 beta (regulatory subunit) antibody. Counter stained with hematoxylin.
ICC staining PKA 2 beta (regulatory subunit) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PKA 2 beta (regulatory subunit) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PKA 2 beta (regulatory subunit) in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of N2A cells with PKA 2 beta (regulatory subunit) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The second messenger cyclic AMP mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. One of several regulatory subunits, p-PKA IIβ reg (cAMP-dependent protein kinase type II-beta regulatory subunit), also known as PRKAR2B, is a 418 amino acid protein that is phosphorylated by the activated catalytic chain. p-PKA IIβ reg knockout mice exhibit diminished white adipose tissue and were protected against diet-induced obesity and fatty livers,as well as markedly reduced leptin mRNA. Also playing a role in the immune response, p-PKA IIβ reg suppresses CREB transcriptional activity and down-regulates IL-2 production in T-lymphocytes.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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