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Choline Acetyltransferase Rabbit mAb#49528

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Product Detail

Product NameCholine Acetyltransferase Rabbit mAb

Clone No.JA67-11

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, IP, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen DescA synthesized peptide derived from human CHAT

ConjugateUnconjugated

Other NamesAcetyl CoA choline O acetyltransferase antibody
Acetyl CoA:choline O acetyltransferase antibody
ChAT antibody
CHOACTase antibody
Choline acetylase antibody
choline acetyltransferase antibody
Choline O acetyltransferase antibody
Choline O-acetyltransferase antibody
CLAT_HUMAN antibody
CMS1A antibody
CMS1A2 antibody
EC 2.3.1.6 antibody
OTTHUMP00000019583 antibody
OTTHUMP00000019584 antibody

Accession NoSwiss-Prot#:P28329

Uniprot P28329

Gene ID 1103;

Calculated MW82 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200
IP 1:20-1:50
FC 1:20-1:100
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.
ICC staining Cho
Line Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Lane1 Mouse brain tissue lysate Use the Antibody at 1:2K dilution for 1 hour at room temperature.

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

If you have published an article using product 49528, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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