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Glucose 6 Phosphate Dehydrogenase Rabbit mAb#49549

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Product Detail

Product NameGlucose 6 Phosphate Dehydrogenase Rabbit mAb

Clone No.JA31-40

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesG6PD antibody
G6PD_HUMAN antibody
G6PD1 antibody
G6pdx antibody
Glucose 6 phosphate 1 dehydrogenase antibody
Glucose 6 phosphate dehydrogenase antibody
Glucose 6 phosphate dehydrogenase, G6PD antibody
Glucose-6-phosphate 1-dehydrogenase antibody
MET19 antibody
POS10 antibody
Zwf1p antibody

Accession NoSwiss-Prot#:P11413

Uniprot P11413

Gene ID 2539;

Calculated MW59 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.
ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Glucose 6 Phosphate Dehydrogenase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Glucose-6-phosphate 1-dehydrogenase (G6PD) plays an important role in the pentose phosphate pathway. It is a member of the glucose-6-phosphate dehydrogenase family of proteins. G6PD is a ubiquitous enzyme that produces pentose sugars for nucleic acid synthesis, but is also involved in carbohydrate degradation, as it is one of the main producers of NADPH reducing power. G6PD has NADP as a co-factor and structural element. It can be found as a homodimer or homotetramer, and is primarily detected in lymphoblasts, granulocytes and sperm. Defects in G6PD can cause chronic non-spherocytic hemolytic anemia (CNSHA), especially in areas in which malaria is an epidemic. Individuals with a high level of G6PD-deficiency are at higher risk of acute hemolytic attacks.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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