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NFAT2 Rabbit mAb#49555

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Product Detail

Product NameNFAT2 Rabbit mAb

Clone No.JA81-03

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namescytoplasmic 1 antibody
MGC138448 antibody
NF ATc antibody
NF ATc1 antibody
NF-ATc antibody
NF-ATc1 antibody
NF-ATc1.2 antibody
NFAC1_HUMAN antibody
NFAT 2 antibody
NFAT transcription complex cytosolic component antibody
NFATC 1 antibody
NFATc antibody
NFATc1 antibody
Nuclear factor of activated T cells cytoplasmic 1 antibody
Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
Nuclear factor of activated T cells cytosolic component 1 antibody
nuclear factor of activated T-cells 'c' antibody
Nuclear factor of activated T-cells antibody

Accession NoSwiss-Prot#:O95644

Uniprot O95644

Gene ID 4772;

Calculated MW101 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human skeleton muscle tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
ICC staining NFAT2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining NFAT2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining NFAT2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with NFAT2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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