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Location： Home > Recombinant Rabbit Monoclonal Antibodies > smooth muscle Myosin heavy chain 11 Rabbit mAb

smooth muscle Myosin heavy chain 11 Rabbit mAb#49569

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Product Namesmooth muscle Myosin heavy chain 11 Rabbit mAb

Clone No.JA03-35

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, ICC/IF

Species ReactivityHu, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAAT4 antibody
DKFZp686D10126 antibody
DKFZp686D19237 antibody
FAA4 antibody
FLJ35232 antibody
MGC126726 antibody
MGC32963 antibody
MYH 11 antibody
MYH11 antibody
MYH11_HUMAN antibody
Myosin 11 antibody
Myosin heavy chain 11 antibody
Myosin heavy chain 11 smooth muscle antibody
Myosin heavy chain antibody
Myosin heavy chain smooth muscle isoform antibody
Myosin heavy polypeptide 11 smooth muscle antibody
Myosin-11 antibody
SMHC antibody
SMMHC antibody
smooth muscle isoform antibody
Smooth muscle myosin heavy chain 11 isoform SM2 antibody
Smooth muscle myosin heavy chain isoform SM2 antibody

Accession NoSwiss-Prot#:P35749

Uniprot P35749

Gene ID 4629;

Calculated MW227 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human jejunum tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat trachea tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.
ICC staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining smooth muscle Myosin heavy chain 11 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits, which is expressed by my calcium and calmodulin-dependent phosphorylation of Myosin light chain (MLC) Myosin heavy chains, encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the Speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on th E functional activity of the muscle.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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