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GOLPH2 Rabbit mAb#49571

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Product Detail

Product NameGOLPH2 Rabbit mAb

Clone No.JA63-03

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesbA379P1.3 antibody
C9orf155 antibody
Chromosome 9 open reading frame 155 antibody
Golgi membrane protein 1 antibody
Golgi membrane protein GP73 antibody
Golgi phosphoprotein 2 antibody
Golgi protein 73 kD antibody
Golgi protein 73kD antibody
GOLM 1 antibody
GOLM1 antibody
GOLM1_HUMAN antibody
GOLPH 2 antibody
GOLPH2 antibody
GP 73 antibody
GP73 antibody
PSEC0257 antibody

Accession NoSwiss-Prot#:Q8NBJ4

Uniprot Q8NBJ4

Gene ID 51280;

Calculated MW100 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50


Western blot analysis of GOLPH2 on SH-SY5Y cell lysate using anti-GOLPH2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GOLPH2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-GOLPH2 antibody. Counter stained with hematoxylin.
ICC staining GOLPH2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GOLPH2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GOLPH2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with GOLPH2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
GP73 (also known as Golgi phosphoprotein 2, GOLPH 2 or Golgi membrane protein), is a widely expressed, epithelial-specific, type II transmembrane protein which resides in the Golgi apparatus, where it is responsible for the posttranslational modification of proteins produced in the Rough ER while assisting in the transport of proteins through the Golgi. The human GP73 gene has been mapped within a BAC and localized to chromosome 9q21.33. GP73 levels rise in those who have been diagnosed with acute and chronic liver diseases.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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