Product Detail
Product NameCDT1 Rabbit mAb
Clone No.JA30-81
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, IHC, IP
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesCDT 1 antibody
cdt1 antibody
CDT1_HUMAN antibody
Chromatin licensing and DNA replication factor 1 antibody
DNA replication factor antibody
DNA replication factor Cdt1 antibody
Double parked antibody
Double parked Drosophila homolog of antibody
Double parked homolog antibody
DUP antibody
Retroviral integration site 1 antibody
Retroviral integration site 2 antibody
Retroviral integration site1 antibody
Retroviral integration site2 antibody
RIS 2 antibody
RIS2 antibody
Accession NoSwiss-Prot#:Q9H211
Uniprot
Q9H211
Gene ID
81620;
Calculated MW60 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
IHC: 1:25-1:50
Western blot analysis of CDT1 on Hela cell using anti-CDT1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CDT1 antibody. Counter stained with hematoxylin.
Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1.
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