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PKC epsilon Rabbit mAb#49589

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Product Detail

Product NamePKC epsilon Rabbit mAb

Clone No.JA38-21

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesKPCE_HUMAN antibody
MGC125656 antibody
MGC125657 antibody
nPKC epsilon antibody
nPKC-epsilon antibody
PKCE antibody
Pkcea antibody
PRKCE antibody
Protein kinase C epsilon antibody
Protein kinase C epsilon type antibody

Accession NoSwiss-Prot#:Q02156

Uniprot Q02156

Gene ID 5581;

Calculated MW84 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1,000 dilution.
Positive control��
Lane1: Jurkat
Lane2: MCF-7
Lane3: HT-29
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PKC epsilon antibody. Counter stained with hematoxylin.
ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PKC epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY5Y cells with PKC epsilon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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