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Calpain 2 Rabbit mAb#49593

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Product Detail

Product NameCalpain 2 Rabbit mAb

Clone No.JA43-41

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCalcium activated neutral proteinase 2 antibody
Calcium activated neutral proteinase antibody
Calcium-activated neutral protease 2, catalytic subunit antibody
Calcium-activated neutral proteinase 2 antibody
CALP80 antibody
Calpain 2 (m/II) large subunit antibody
Calpain 2 catalytic subunit antibody
Calpain 2 large catalytic subunit antibody
Calpain 2 large subunit antibody
Calpain 2, large [catalytic] subunit antibody
Calpain large polypeptide L2 antibody
Calpain M type antibody
Calpain M-type antibody
Calpain-2 catalytic subunit antibody
Calpain-2 large subunit antibody
Calpain2 antibody
CAN2_HUMAN antibody
CANP 2 antibody
CANP L2 antibody
CANP2 antibody
CANPL 2 antibody
CANPL2 antibody
CANPml antibody
Capa2 antibody
CAPN 2 antibody
CAPN2 antibody
FLJ39928 antibody
M calpain antibody
M calpin antibody
M type antibody
M-calpain antibody
mCANP antibody
Millimolar calpain antibody
Millimolar-calpain antibody

Accession NoSwiss-Prot#:P17655

Uniprot P17655

Gene ID 824;

Calculated MW80 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200


Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.

ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.

If you have published an article using product 49593, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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