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Location： Home > Recombinant Rabbit Monoclonal Antibodies > DDX5 Rabbit mAb

DDX5 Rabbit mAb#49625

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Product Detail

Product NameDDX5 Rabbit mAb

Clone No.JM52-30

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesATP dependent RNA helicase DDX5 antibody
DDX 5 antibody
Ddx5 antibody
DDX5_HUMAN antibody
DEAD (Asp Glu Ala Asp) box helicase 5 antibody
DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
DEAD box 5 antibody
DEAD box protein 5 antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
G17P1 antibody
HELR antibody
HLR1 antibody
HUMP68 antibody
P68 antibody
p68 RNA helicase antibody
Probable ATP dependent RNA helicase DDX5 antibody
Probable ATP-dependent RNA helicase DDX5 antibody
RNA helicase p68 antibody

Accession NoSwiss-Prot#:P17844

Uniprot P17844

Gene ID 1655;

Calculated MW69/60 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution. Positive control��
Lane1: PC-12
Lane2: MEF
Lane3: SH-SY5Y
Lane4: NIH-3T3
Lane5: Mouse brain
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.
ICC staining DDX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DDX5 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DDX5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY5Y cells with DDX5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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