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Skp1 Rabbit mAb#49631

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Product Detail

Product NameSkp1 Rabbit mAb

Clone No.JM27-37

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCyclin A/CDK2 associated p19 antibody
Cyclin A/CDK2 associated protein p19 antibody
Cyclin-A/CDK2-associated protein p19 antibody
EMC19 antibody
MGC34403 antibody
OCP 2 antibody
OCP II antibody
OCP II protein antibody
OCP-2 antibody
OCP-II antibody
OCP2 antibody
OCPII antibody
Organ of Corti protein 2 antibody
Organ of Corti protein II antibody
OTTHUMP00000159379 antibody
OTTHUMP00000159380 antibody
OTTHUMP00000228275 antibody
OTTHUMP00000228276 antibody
OTTHUMP00000228278 antibody
OTTHUMP00000228279 antibody
OTTHUMP00000228281 antibody
p19A antibody
p19skp1 antibody
RNA polymerase II elongation factor like protein antibody
RNA polymerase II elongation factor like protein OCP2 antibody
RNA polymerase II elongation factor-like protein antibody
S phase kinase associated protein 1 antibody
S phase kinase associated protein 1A antibody
S phase kinase associated protein 1A p19A antibody
S-phase kinase-associated protein 1 antibody
SIII antibody
Skp 1 antibody
SKP 1A antibody
skp1 antibody
SKP1_HUMAN antibody
SKP1A antibody
TCEB1L antibody
Transcription Elongation Factor B 1 like antibody
Transcription elongation factor B antibody
Transcription elongation factor B SIII polypeptide 1 like antibody

Accession NoSwiss-Prot#:P63208

Uniprot P63208

Gene ID 6500;

Calculated MW19 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500

ICC: 1:50-1:100

FC: 1:50-1:100
Western blot analysis of Skp1 on PC-12 cell using anti-Skp1 antibody at 1/1,000 dilution.
ICC staining Skp1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Skp1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Skp1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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