Product Detail
Product NameBMAL1 Rabbit mAb
Clone No.JM17-34
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesARNT like protein 1 brain and muscle antibody
Arntl antibody
Aryl hydrocarbon receptor nuclear translocator like antibody
Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody
Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody
Basic helix loop helix PAS orphan MOP3 antibody
Basic helix loop helix PAS protein MOP3 antibody
Basic-helix-loop-helix-PAS protein MOP3 antibody
bHLH PAS protein JAP3 antibody
bHLH-PAS protein JAP3 antibody
bHLHe5 antibody
BMAL 1 antibody
BMAL1_HUMAN antibody
BMAL1c antibody
Brain and muscle ARNT like 1 antibody
Brain and muscle ARNT-like 1 antibody
CG8727 PA antibody
Class E basic helix-loop-helix protein 5 antibody
cycle antibody
JAP 3 antibody
JAP3 antibody
Member of PAS protein 3 antibody
Member of PAS superfamily 3 antibody
MGC47515 antibody
MOP 3 antibody
MOP3 antibody
PAS domain-containing protein 3 antibody
PASD 3 antibody
PASD3 antibody
TIC antibody
Accession NoSwiss-Prot#:O00327
Uniprot
O00327
Gene ID
406;
Calculated MW68 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
Western blot analysis of BMAL1 on different lysates using anti-BMAL1 antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3
Lane 2: Rat brain
Lane 3: Mouse spleen
Lane 4: Hela
ICC staining BMAL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining BMAL1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Transcriptional activator which forms a core component of the circadian clock. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway.
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