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Location： Home > Recombinant Rabbit Monoclonal Antibodies > SMYD3 Rabbit mAb

SMYD3 Rabbit mAb#49647

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Product Detail

Product NameSMYD3 Rabbit mAb

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesbA74P14.1 (novel protein) antibody
bA74P14.1 antibody
FLJ21080 antibody
histone lysine N methyltransferase SMYD3 antibody
KMT3E antibody
MGC104324 antibody
SET and MYND domain containing 3 antibody
SET and MYND domain containing protein 3 antibody
SET and MYND domain-containing protein 3 antibody
SMYD 3 antibody
Smyd3 antibody
SMYD3 protein antibody
SMYD3_HUMAN antibody
Zinc finger MYND domain containing 1 antibody
Zinc finger MYND domain containing protein 1 antibody
Zinc finger MYND domain-containing protein 1 antibody
Zinc finger protein subfamily 3A MYND domain containing 1 antibody
Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
ZMYND 1 antibody
ZMYND1 antibody
ZNFN3A1 antibody

Accession NoSwiss-Prot#:Q9H7B4

Uniprot Q9H7B4

Gene ID 64754;

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution. Positive control�� Lane1: Rat kidney
Lane2: PC-12
Lane3: Hela
Lane4: Mouse spleen
Lane5: NIH-3T3
Lane6: Rat spleen
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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