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Nogo B Rabbit mAb#49653

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Product Detail

Product NameNogo B Rabbit mAb

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names1110020G17Rik antibody
AA407876 antibody
AA409940 antibody
AA960376 antibody
ASY antibody
C130026I10Rik antibody
Foocen antibody
Glut4 vesicle 20 kDa protein antibody
Human NogoA antibody
Kiaa0886 antibody
KIAA4153 antibody
MGC116054 antibody
MGC139261 antibody
mKIAA0886 antibody
mKIAA4153 antibody
My043 protein antibody
Nbla00271 antibody
Nbla10545 antibody
Neurite growth inhibitor 220 antibody
Neurite Growth Inhibitor 220, included antibody
Neurite outgrowth inhibitor antibody
Neuroendocrine-specific protein antibody
Neuroendocrine-specific protein C homolog antibody
NI-250 antibody
NI220/250 antibody
Nogo A antibody
NOGO antibody
Nogo B antibody
Nogo C antibody
Nogo protein antibody
NOGOC antibody
NSP antibody
NSP-CL antibody
rat N antibody
Reticulon 4 antibody
Reticulon 5 antibody
Reticulon-4 antibody
Reticulon-5 antibody
RTN X antibody
RTN-x antibody
Rtn4 antibody
Rtn4 reticulon 4 antibody
RTN4-A antibody
RTN4-B1 antibody
RTN4-B2 antibody
RTN4-C antibody
RTN4_HUMAN antibody
Vp20 antibody

Accession NoSwiss-Prot#:Q9NQC3

Uniprot Q9NQC3

Gene ID 57142;

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of Nogo on mouse skeletal muscle tissue (1) and Hela cell (2) lysate using anti-Nogo antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Nogo antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Nogo antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nogo antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Nogo antibody. Counter stained with hematoxylin.
ICC staining Nogo in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Nogo in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Nogo in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Nogo antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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