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Galectin 1 Rabbit mAb#49673

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Product Detail

Product NameGalectin 1 Rabbit mAb

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Rt, Ms

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names14 kDa laminin-binding protein antibody
14 kDa lectin antibody
Beta galactoside binding lectin antibody
Beta galactoside binding lectin L 14 I antibody
beta galactoside binding protein antibody
Beta-galactoside-binding lectin L-14-I antibody
DKFZp686E23103 antibody
Gal 1 antibody
Gal-1 antibody
GAL1 antibody
Galaptin antibody
Galbp antibody
Galectin antibody
Galectin-1 antibody
Galectin1 antibody
GBP antibody
HBL antibody
HLBP14 antibody
HPL antibody
L 14.5 antibody
L-14.5 antibody
L14 antibody
Lactose binding lectin 1 antibody
Lactose-binding lectin 1 antibody
Lect14 antibody
Lectin galactoside binding soluble 1 antibody
Lectin galactoside-binding soluble 1 antibody
LEG1_HUMAN antibody
LGALS 1 antibody
LGALS1 antibody
Lgals1 lectin galactose binding soluble 1 antibody
MAPK activating protein MP12 antibody
Putative MAPK activating protein MP12 antibody
Putative MAPK-activating protein PM12 antibody
S Lac lectin 1 antibody
S-Lac lectin 1 antibody

Accession NoSwiss-Prot#:P09382

Uniprot P09382

Gene ID 3956;

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:400-1:800
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of Galectin 1 on HL-60 cell (1) and human skeletal muscle tissue (2) lysate using anti-Galectin 1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Galectin 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. Counter stained with hematoxylin.
ICC staining Galectin 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH-3T3 cells with Galectin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. Specifically, Galectin-1 is an autocrine regulator of cell-proliferation that plays a role in the maintenance of G0 and in the control of G2 traverse. Galectin-1, also known as LGALS1, is the protein product of a single gene linked to human chromosome 22q13.1. The Galectin-1 protein contains 135 amino acids, a single internal EcoRI site and a polyadenylation signal. Galectin-1 can localize to both intracellular and extracellular space. Galectin-1 is expressed in human placenta, human lung, HL-6, HepG2 and CEM cells.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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