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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Vinculin Rabbit mAb

Vinculin Rabbit mAb#49684

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Product Detail

Product NameVinculin Rabbit mAb

Clone No.JM42-43

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB;IHC;IF/ICC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic peptide within human Vinculin

ConjugateUnconjugated

Other NamesCMD1W antibody
CMH15 antibody
Epididymis luminal protein 114 antibody
HEL114 antibody
Metavinculin antibody
MV antibody
MVCL antibody
OTTHUMP00000019861 antibody
OTTHUMP00000019862 antibody
VCL antibody
VINC antibody
VINC_HUMAN antibody
Vinculin antibody

Accession NoSwiss-Prot#:P18206

Uniprot P18206

Gene ID 7414;

Calculated MW124 kDa

Concentration1ug/ul

Formulation1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Application Details

WB 1:5000-1:20000;
IF-Cell 1:200-1:1000;
IF-Tissue 1:50-1:4000;
IHC-P 1:200-1:20000

Western blot analysis of Vinculin on different lysates with Vinculin antibody at 1/20,000 dilution.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: C6 cell lysate (15 µg/Lane)
Lane 5: Mouse spleen tissue lysate (20 µg/Lane)
Lane 6: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 124 kDa
Observed band size: 124 kDa
Exposure time: 1 minute 21 seconds;
4-20% SDS-PAGE gel.
Immunocytochemistry analysis of HeLa cells labeling Vinculin with Vinculin antibody at 1/1,000 dilution.
Flow cytometric analysis of HeLa cells labeling Vinculin.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Vinculin antibody at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Vinculin antibody at 1/20,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Vinculin antibody at 1/20,000 dilution.
Immunocytochemistry analysis of NIH/3T3 cells labeling Vinculin with Vinculin antibody at 1/250 dilution.
Immunocytochemistry analysis of C6 cells labeling Vinculin with Vinculin antibody at 1/250 dilution.

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

If you have published an article using product 49684, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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