Product Detail
Product NameEg5 Rabbit mAb
Clone No.JU83-16
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB,IP
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesEG5 antibody
HKSP antibody
KIF11 antibody
KIF11_HUMAN antibody
Kinesin family member 11 antibody
Kinesin like protein 1 antibody
Kinesin-like protein 1 antibody
Kinesin-like protein KIF11 antibody
Kinesin-like spindle protein HKSP antibody
Kinesin-related motor protein Eg5 antibody
KNSL1 antibody
MCLMR antibody
Thyroid receptor-interacting protein 5 antibody
TR-interacting protein 5 antibody
TRIP-5 antibody
TRIP5 antibody
Accession NoSwiss-Prot#:P52732
Uniprot
P52732
Gene ID
3832;
Calculated MW119 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
IP: 1:10-1:50
Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution.
Positive control:
Lane 1: 293
Lane 2: A431
Lane 3: Jurkat
Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.
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