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Location： Home > Recombinant Rabbit Monoclonal Antibodies > alpha Actinin 4 Rabbit mAb

alpha Actinin 4 Rabbit mAb#49693

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Product Detail

Product Namealpha Actinin 4 Rabbit mAb

Clone No.JU20-23

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,ICC,IF,IP,IHC,FC

Species ReactivityHu, Ms, Rt

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Namesactinin 4 antibody
Actinin alpha 4 antibody
actinin4 antibody
ACTN 4 antibody
ACTN4 antibody
ACTN4_HUMAN antibody
Alpha-actinin-4 antibody
DKFZp686K23158 antibody
F actin cross linking protein antibody
F-actin cross-linking protein antibody
Focal segmental glomerulosclerosis 1 antibody
FSGS 1 antibody
FSGS antibody
FSGS1 antibody
Non muscle alpha actinin 4 antibody
Non-muscle alpha-actinin 4 antibody

Accession NoSwiss-Prot#:O43707

Uniprot O43707

Gene ID 81;

Calculated MW105 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH-3T3
Lane 4: Rat liver tissue
Lane 5: A431
Lane 6: HepG2
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining alpha Actinin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining alpha Actinin 4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A549 cells with alpha Actinin 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. are actually sensitive to the Profilin proteins in these foods.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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