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NDUFS3 Rabbit mAb#49695

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Product Detail

Product NameNDUFS3 Rabbit mAb

Clone No.JU10-32

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,IHC,ICC,IF,IP

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesCI 30 antibody
CI 30KD antibody
CI-30kD antibody
Complex I 30KD antibody
Complex I 30kDa subunit antibody
COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody
Complex I-30kD antibody
mitochondrial antibody
NADH coenzyme Q reductase antibody
NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody
NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody
NADH dehydrogenase ubiquinone 30 kDa subunit antibody
NADH-ubiquinone oxidoreductase 30 kDa subunit antibody
NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody
NDUFS3 antibody
NDUS3_HUMAN antibody

Accession NoSwiss-Prot#:O75489

Uniprot O75489

Gene ID 4722;

Calculated MW25 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500
IHC: 1:50
ICC: 1:50
IP: 1:10-1:50
Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.
ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
NDUFS3 (NADH dehydrogenase ubiquinone iron-sulfur protein 3) is one of about 45 subunits comprising complex I of the oxidative phosphorylation electron transport chain. The multisubunit NADH: ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of the mitochondria. NDUFS3 is the last subunit of the seven subunits that make up the core of complex I. Through use of chaotropic agents, complex I can be separated into three different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction includes NDUFS1-7. NDUFS3 contains a highly conserved casein kinase II phosphorylation site. Mutations in the NDUFS3 gene may cause optic atrophy, Leigh syndrome and complex I deficiency.

If you have published an article using product 49695, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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