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HPRT Rabbit mAb#49696

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Product Detail

Product NameHPRT Rabbit mAb

Clone No.JU03-26

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,IHC,IP

Species ReactivityHu, Ms, Rt, Zebrafish

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesHGPRT antibody
HGPRTase antibody
HPRT 1 antibody
HPRT_HUMAN antibody
HPRT1 antibody
Hypoxanthine guanine phosphoribosyltransferase antibody
Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody
Hypoxanthine phosphoribosyltransferase 1 antibody
Hypoxanthine-guanine phosphoribosyltransferase antibody

Accession NoSwiss-Prot#:P00492

Uniprot P00492

Gene ID 3251;

Calculated MW25 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200

IP: 1:10-1:50
Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: A431
Lane 3: MCF-7
Lane 4: 293
Lane 5: Rat kidney
Lane 6: Rat brain
Lane 7: Mouse testis
Lane 8: Mouse colon
Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.
HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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