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Location： Home > Recombinant Rabbit Monoclonal Antibodies > USP13 Rabbit mAb

USP13 Rabbit mAb#49702

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Product Detail

Product NameUSP13 Rabbit mAb

Clone No.JU72-38

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,ICC/IF,FC,IP

Species ReactivityHu, Ms

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesDeubiquitinating enzyme 13 antibody
Isopeptidase T 3 antibody
Isopeptidase T-3 antibody
Isopeptidase T3 antibody
ISOT 3 antibody
ISOT-3 antibody
ISOT3 antibody
Ubiquitin carboxyl terminal hydrolase 13 antibody
Ubiquitin carboxyl-terminal hydrolase 13 antibody
Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
Ubiquitin specific peptidase 13 antibody
Ubiquitin specific processing protease 13 antibody
Ubiquitin specific protease 13 antibody
Ubiquitin thioesterase 13 antibody
Ubiquitin thiolesterase 13 antibody
Ubiquitin-specific-processing protease 13 antibody
UBP13 antibody
UBP13_HUMAN antibody
USP 13 antibody
USP13 antibody

Accession NoSwiss-Prot#:Q92995

Uniprot Q92995

Gene ID 8975;

Calculated MW97 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000

ICC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of USP13 on mouse testis tissue lysate using anti-USP13 antibody at 1/500 dilution.
ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining USP13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining USP13 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY5Y cells with USP13 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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