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macroH2A.1 Rabbit mAb#49703

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Product NamemacroH2A.1 Rabbit mAb

Clone No.JU22-83

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB,ICC/IF,IHC

Species ReactivityHu, Ms, Rt

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesCore histone macro h2a.1 antibody
Core histone macro-H2A.1 antibody
H2A histone family member Y antibody
H2A.y antibody
H2A/y antibody
H2AF12M antibody
H2AFJ antibody
H2afy antibody
H2AY_HUMAN antibody
Histone H2A.Y antibody
Histone macroH2A1 antibody
Histone macroH2A1.1 antibody
Histone macroH2A1.2 antibody
Macroh2a1 antibody
MACROH2A1.1 antibody
MacroH2A1.2 antibody
Medulloblastoma antigen MU MB 50.205 antibody
Medulloblastoma antigen MU-MB-50.205 antibody
mH2a antibody
mH2A1 antibody

Accession NoSwiss-Prot#:O75367

Uniprot O75367

Gene ID 9555;

Calculated MW40 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution. Positive control:
Lane 1: Hela
Lane 2: Mouse placenta tissue
Lane 3: MCF-7
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
ICC staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining macroH2A.1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining macroH2A.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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