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Location： Home > Recombinant Rabbit Monoclonal Antibodies > GPX4 Rabbit mAb

GPX4 Rabbit mAb#49731

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Product Detail

Product NameGPX4 Rabbit mAb

Clone No.JU11-31

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,IHC

Species ReactivityHu, Ms, Rt, Zebrafish

Immunogen DescSynthetic peptide within Human GPX4

ConjugateUnconjugated

Other NamesGlutathione peroxidase 4 antibody
GPX 4 antibody
GPX-4 antibody
GPX4 antibody
GPX4_HUMAN antibody
GSHPx-4 antibody
MCSP antibody
mitochondrial antibody
PHGPx antibody
Phospholipid hydroperoxidase antibody
Phospholipid hydroperoxide glutathione peroxidase antibody
Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
snGPx antibody
snPHGPx antibody
Sperm nucleus glutathione peroxidase antibody

Accession NoSwiss-Prot#:P36969

Uniprot P36969

Gene ID 2879;

Calculated MW22 kDa Clone number: JU11-31

Concentration1ug/ul

Formulation1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB 1:10000;
IHC-P 1:1000-1:5000

Western blot analysis of GPX4 on different lysates with GPX4 antibody at 1/10,000 dilution
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: U-937 cell lysate (20 µg/Lane)
Lane 5: HEK-293 cell lysate (20 µg/Lane)
Lane 6: Mouse testis tissue lysate (20 µg/Lane)
Lane 7: Rat testis tissue lysate (20 µg/Lane)
Lane 8: Mouse kidney tissue lysate (20 µg/Lane)
Lane 9: Rat kidney tissue lysate (20 µg/Lane)
Lane 10: Mouse brain tissue lysate (20 µg/Lane)
Lane 11: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Western blot analysis of GPX4 on zebrafish tissue lysates with GPX4 antibody at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 17 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with GPX4 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with GPX4 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with GPX4 antibody at 1/5,000 dilution.

Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers.

If you have published an article using product 49731, please notify us so that we can cite your literature.

et al,Linoleic Acid–Glucosamine Hybrid for Endogenous Iron-Activated Ferroptosis Therapy in High-Grade Serous Ovarian Cancer. In Mol Pharm on 2022 Sep 5 by Ying Chen, Xiaoming Liao, et al..PMID:35939328 , (2022),
PMID: 35939328
et al,Classical prescription Daqinjiao decoction inhibit cerebral ischemia/reperfusion induced necroptosis and ferroptosis through multiple mechanisms. , (2025),
PMID: 39736347

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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