Product Detail
Product NameDAP12 Rabbit mAb
Clone No.JG38-70
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB,IHC,FC
Species ReactivityHu
Immunogen DescSynthetic peptide within human DAP12 aa 60-100.
ConjugateUnconjugated
Other NamesDAP 12 antibody
DAP12 antibody
DNAX activation protein 12 antibody
DNAX-activation protein 12 antibody
KAR-associated protein antibody
KARAP antibody
Killer activating receptor associated protein antibody
Killer-activating receptor-associated protein antibody
PLOSL antibody
TYOBP_HUMAN antibody
TYRO protein tyrosine kinase binding protein antibody
TYRO protein tyrosine kinase-binding protein antibody
TYROBP antibody
Accession NoSwiss-Prot#:O43914
Uniprot
O43914
Gene ID
7305;
Calculated MW12 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.
If you have published an article using product 49944, please notify us so that we can cite your literature.
et al,Engineered circular RNA-based DLL3-targeted CAR-T therapy for small cell lung cancer.
, (2025),
PMID:
40075480
Yes
