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OPN Polyclonal Antibody#49995

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Product Detail

Product NameOPN Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous OPN

Immunogen DescSynthetic peptide from human protein at AA range: 91-140

ConjugateUnconjugated

Other NamesOsteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

Accession NoSwiss_Prot:P10451
Gene_ID:6696

Uniprot P10451

Gene ID 6696

Calculated MW35kDa

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20°C/1 year

Application Details

WB dilution: 1:500-2000

IHC dilution: 1:50-200

 

Western Blot analysis of HepG2 cell.
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer, antibody was diluted at 1:100
Immunohistochemical analysis of paraffin-embedded Human-brain, antibody was diluted at 1:100

Secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

If you have published an article using product 49995, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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