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Location： Home > Phospho Antibodies > Rb2 p130 (Phospho-Thr986) Rabbit mAb

Rb2 p130 (Phospho-Thr986) Rabbit mAb#52095

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Product NameRb2 p130 (Phospho-Thr986) Rabbit mAb

Clone No.S06-9B3

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman,Mouse

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Thr986 of human p130

ConjugateUnconjugated

Other NamesRb2; P130

Accession NoSwiss-Prot:Q08999
GeneID:5934

Uniprot Q08999

Gene ID 5934

Calculated MWPredicted band size: 128 kDa

Sdspage MWObserved band size: 130kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
All lanes : Rb2 p130 (Phospho-Thr986) Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : K562 treated with Lambda Protein Phosphatase for 30min whole cell lysates/br>Lane 3 : JK whole cell lysates
Lane 4 : C2C12 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 128 kDa
Observed band size: 130kDa

Exposure time: 3 seconds
Swiss-Prot Acc.Q08999.Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

If you have published an article using product 52095, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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