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EpCAM Rabbit mAb#52268

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Product Detail

Product NameEpCAM Rabbit mAb

Clone No.S08-8D1

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB IHC IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant protein of human EpCAM

ConjugateUnconjugated

Other NamesESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

Accession NoSwiss-Prot:P16422
GeneID:4072

Uniprot P16422

Gene ID 4072

Calculated MWCalculated MW: 35 kDa; Observed MW: 35/39 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000; IHC: 1/500; ICC/IF: 1/20;
Western blot detection of EpCAM in MCF7,HCT116,T47D,Hela cell lysates using EpCAM Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:40kDa.
Immunofluorescence of EpCAM (green) in MCF-7 using EpCAM antibody at dilution 1/2, and DAPI(blue)
Immunohistochemistry of EpCAM in paraffin-embedded Human breast cancer tissue using EpCAM Rabbit mAb at dilution 1/50
Swiss-Prot Acc.P16422.May act as a physical homophilic interaction molecule between inTinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

If you have published an article using product 52268, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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