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FOXO3A (Phospho-Ser253) Rabbit mAb#52289

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Product NameFOXO3A (Phospho-Ser253) Rabbit mAb

Clone No.S06-2G6

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser253 of human FOXO3A

ConjugateUnconjugated

Other NamesFOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Accession NoSwiss-Prot:O43524
GeneID:2309

Uniprot O43524

Gene ID 2309

Calculated MWCalculated MW: 71 kDa; Observed MW: 82 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000
Western blot detection of Phospho-FOXO3A (Ser253) in K562,C6 cell lysates using Phospho-FOXO3A (Ser253) Rabbit mAb(1:1000 diluted).Predicted band size:71kDa.Observed band size:82kDa.
Swiss-Prot Acc.O43524.Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' (PubMed:21329882). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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