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CD147 Rabbit mAb#52667

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Product Detail

Product NameCD147 Rabbit mAb

Clone No.S04-5B3

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human CD147

ConjugateUnconjugated

Other NamesOK; 5F7; TCSF; CD147; EMMPRIN

Accession NoSwiss-Prot:P35613
GeneID:682

Uniprot P35613

Gene ID 682

Calculated MWCalculated MW:42 kDa,Observed MW:50 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000-1/5000 IHC: 1/50-1/200
Western blot detection of CD147 in Hela,Ramos cell lysates using CD147 Rabbit mAb(1:1000 diluted).Predicted band size:42kDa.Observed band size:42kDa.
Immunohistochemistry of CD147 in paraffin-embedded Human colon cancer tissue using CD147 Rabbit mAb at dilution 1/50
Essential for normal retinal maturation and development (By similarity).Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687).In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687).May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).

If you have published an article using product 52667, please notify us so that we can cite your literature.

et al,Effect of silencing CD147 on glycolysis in prostate cancer LNCaP cells , (2023),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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