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AKT1 (Phospho-Thr450) Rabbit mAb#52689

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Product Detail

Product NameAKT1 (Phospho-Thr450) Rabbit mAb

Clone No.S07-4A7

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Thr450 of human AKT1

ConjugateUnconjugated

Other NamesAKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Accession NoSwiss-Prot:P31749
GeneID:207

Uniprot P31749

Gene ID 207

Calculated MWPredicted band size: 56 kDa

Sdspage MWObserved band size: 56 kDa

Concentration0.3 mg/ml

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : AKT1 (Phospho-Thr450) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : MCF7 whole cell lysates
Lane 4 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for AKT1 (Phospho-T450) using 52689 at 1/100 dilution in immunohistochemical analysis.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

If you have published an article using product 52689, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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