Product Detail
Product NameAMPK alpha 1 (Phospho-Ser496) Rabbit mAb
Clone No.S01-3B7
Host SpeciesRecombinant Rabbit
ClonalityMonoclonal
IsotypeIgG
PurificationAffinity Purified
ApplicationsWB IF
Species ReactivityHuman
Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser496 of human AMPK alpha 1
ConjugateUnconjugated
Other NamesAMPK; AMPKa1
Accession NoSwiss-Prot:Q13131
GeneID:5562
Uniprot
Q13131
Gene ID
5562
Calculated MWCalculated MW:64 kDa,Observed MW:64 kDa
Concentration0.3 mg/ml
Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Application Details
WB: 1/10001/50
Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using AMPK alpha 1 (phospho-Ser496) Rabbit mAb(1:1000 diluted).Predicted band size:64kDa.Observed band size:64kDa.
Immunocytochemistry of AMPK alpha 1 (phospho-Ser496) (green) in hela using AMPK alpha 1 (phospho-Ser496) Rabbit mAb at dilution 1/50, and DAPI(blue)
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357).In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:17307971, PubMed:17712357).AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:17307971, PubMed:17712357).Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity).Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2) (PubMed:34077757).
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