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Phospho Antibodies > Cyclin B1 (Phospho-Ser126) Rabbit mAb
Cyclin B1 (Phospho-Ser126) Rabbit mAb#52692
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All lanes : Cyclin B1 (Phospho-Ser126) Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysatesLane 2 : Ramos whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 48 kDa Observed band size: 55 kDaExposure time: 9 seconds
All lanes : Cyclin B1 (Phospho-Ser126) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HT1080 cell lysate
Lane 2 : Cyclin B1 (Ser126) knockout HT1080 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil cancer tissue stained for Cyclin B1 (Phospho-Ser126) using 52692 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Cyclin B1 (Phospho-Ser126) antibody (52692)
ICC/IF staining of Cyclin B1 (Phospho-Ser126) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 52692 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
