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Location： Home > Phospho Antibodies > Filamin A/B (Phospho-Ser2152/Ser2107) Rabbit mAb

Filamin A/B (Phospho-Ser2152/Ser2107) Rabbit mAb#52694

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Product NameFilamin A/B (Phospho-Ser2152/Ser2107) Rabbit mAb

Clone No.S02-7C0

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser2152 of human Filamin A

ConjugateUnconjugated

Other NamesFLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280

Accession NoSwiss-Prot:O75369
GeneID:2316

Uniprot O75369

Gene ID 2316

Calculated MWPredicted band size: 281 kDa

Sdspage MWObserved band size: 281 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Filamin A/B (Phospho-Ser2152/Ser2107) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 281 kDa
Observed band size: 281 kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human cholangiocarcinoma tissue stained for Filamin A/B (Phospho-Ser2152/Ser2107) using 52694 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Filamin A/B (Phospho-Ser2152/Ser2107) antibody (52694)

ICC/IF staining of Filamin A/B (Phospho-Ser2152/Ser2107) in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 52694 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.

If you have published an article using product 52694, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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