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Glucocorticoid Receptor (Phospho-Ser226) Rabbit mAb#52695

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Product NameGlucocorticoid Receptor (Phospho-Ser226) Rabbit mAb

Clone No.S02-1H0

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB,IHC

Species ReactivityHu，Rt

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser226 of human Glucocorticoid Receptor

ConjugateUnconjugated

Other NamesGR; GCR; GRL; GCCR; GCRST

Accession NoSwiss-Prot:P04150
GeneID:2908

Uniprot P04150

Gene ID 2908

Calculated MWPredicted band size: 86 kDa

Sdspage MWObserved band size: 94 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : Glucocorticoid Receptor (Phospho-Ser226) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with UV for 15min whole cell
Lane 3 : C6 whole cell lysates
Lane 4 : C6 treated with UV for 15min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 94 kDa

Exposure time: 8 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Glucocorticoid Receptor (Phospho-Ser226) using 52695 at 1/100 dilution in immunohistochemical analysis.
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

If you have published an article using product 52695, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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