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Location： Home > Phospho Antibodies > IRE1 (Phospho-Ser724) Rabbit mAb

IRE1 (Phospho-Ser724) Rabbit mAb#52697

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Product Detail

Product NameIRE1 (Phospho-Ser724) Rabbit mAb

Clone No.S05-6E7

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser724 of human IRE1

ConjugateUnconjugated

Other NamesIRE1b; IRE2p; hIRE2p; IRE1-BETA

Accession NoSwiss-Prot:O75460
GeneID:10595

Uniprot O75460

Gene ID 10595

Calculated MWPredicted band size: 110 kDa

Sdspage MWObserved band size: 110 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
All lanes : IRE1 (Phospho-Ser724) Rabbit mAb at 1/1k dilution
Lane 1 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell
Lane 2 : MCF7 whole cell lysates
Lane 3 : HT29 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 3 seconds
Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875)

If you have published an article using product 52697, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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