Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > MCM2 (Phospho-Ser40) Rabbit mAb

MCM2 (Phospho-Ser40) Rabbit mAb#52698

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameMCM2 (Phospho-Ser40) Rabbit mAb

Clone No.S03-1B3

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser40 of human MCM2

ConjugateUnconjugated

Other NamesBM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN

Accession NoSwiss-Prot:P49736
GeneID:4171

Uniprot P49736

Gene ID 4171

Calculated MWPredicted band size: 102 kDa

Sdspage MWObserved band size: 125 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : MCM2 (Phospho-Ser40) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa

Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for MCM2 (Phospho-Ser40) using 52698 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence MCM2 (Phospho-Ser40) antibody(52698)

ICC/IF staining of MCM2 (Phospho-Ser40) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 52698 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

If you have published an article using product 52698, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors