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Location： Home > Phospho Antibodies > c Met (Phospho-Tyr1349) Rabbit mAb

c Met (Phospho-Tyr1349) Rabbit mAb#52699

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Product Namec Met (Phospho-Tyr1349) Rabbit mAb

Clone No.S05-3D6

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB,IHC

Species ReactivityHuman

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Tyr1349 of human Met

ConjugateUnconjugated

Other NamesHGFR; AUTS9; RCCP2; c-Met; DFNB97

Accession NoSwiss-Prot:P08581
GeneID:4233

Uniprot P08581

Gene ID 4233

Calculated MWPredicted band size: 156 kDa

Sdspage MWObserved band size: 140kDa

Concentration0.3 mg/ml

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : c Met (Phospho-Tyr1349) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 40 ng/ml HGF for 5 min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 140kDa

Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for Phospho-c Met (Tyr1349) using 52699 at 1/100 dilution in immunohistochemical analysis.
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]

If you have published an article using product 52699, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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