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Location： Home > Phospho Antibodies > PKC delta (Phospho-Ser299) Rabbit mAb

PKC delta (Phospho-Ser299) Rabbit mAb#52700

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Product Detail

Product NamePKC delta (Phospho-Ser299) Rabbit mAb

Clone No.S06-4A5

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB,IHC

Species ReactivityHuman,Mouse

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser299 of human PKC delta

ConjugateUnconjugated

Other NamesMAY1; PKCD; ALPS3; CVID9; nPKC-delta

Accession NoSwiss-Prot:Q05655
GeneID:5580

Uniprot Q05655

Gene ID 5580

Calculated MWPredicted band size: 78 kDa

Sdspage MWObserved band size: 78 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : PKC delta (Phospho-Ser299) Rabbit mAb at 1/1k dilution
Lane 1 : HL60 whole cell lysates
Lane 2 : HL60 treated with Lambda Protein Phosphatase for 30min whole cell
Lane 3 : A549 whole cell lysates
Lane 4 : Mouse Brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa

Exposure time: 4 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PKC delta (Phospho-Ser299) using 52700 at 1/100 dilution in immunohistochemical analysis.
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]

If you have published an article using product 52700, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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