Product Detail
Product NameSmad1 (Phospho-Ser463/Ser465) Rabbit mAb
Clone No.S07-4A3
Host SpeciesRecombinant Rabbit
ClonalityMonoclonal
IsotypeIgG
PurificationAffinity Purified
ApplicationsWB
Species ReactivityHu，Rt
Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser463/Ser465 of human Smad1
ConjugateUnconjugated
Other NamesBSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Accession NoSwiss-Prot:Q15797
GeneID:4086
Uniprot
Q15797
Gene ID
4086
Calculated MWPredicted band size: 52 kDa
Sdspage MWObserved band size: 52 kDa
Concentration0.3 mg/ml
Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Application Details
WB: 1:500-1:2000
All lanes : Smad1 (Phospho-Ser463/Ser465) Rabbit mAb at 1/1k dilutionLane 1 : A549 whole cell lysatesLane 2 : A549 treated with Lambda Protein Phosphatase for 30min whole cellLane 3 : U2OS whole cell lysatesLane 4 : C6 whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 52 kDa Observed band size: 52 kDaExposure time: 3 seconds
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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