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Location： Home > Phospho Antibodies > Smad2 (Phospho-Ser250) Rabbit mAb

Smad2 (Phospho-Ser250) Rabbit mAb#52703

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Product Detail

Product NameSmad2 (Phospho-Ser250) Rabbit mAb

Clone No.S02-0D1

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser250 of human Smad2

ConjugateUnconjugated

Other NamesJV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Accession NoSwiss-Prot:Q15796
GeneID:4087

Uniprot Q15796

Gene ID 4087

Calculated MWCalculated MW:52,48 kDa,Observed MW:58,62 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000
Western blot detection of Smad2 (phospho-Ser250) in Hela,CHO-K1,C6 using Smad2 (phospho-Ser250) Rabbit mAb(1:1000 diluted)
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

If you have published an article using product 52703, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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