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Location： Home > Phospho Antibodies > TrkB (Phospho-Tyr705) Rabbit mAb

TrkB (Phospho-Tyr705) Rabbit mAb#52705

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Product Detail

Product NameTrkB (Phospho-Tyr705) Rabbit mAb

Clone No.S04-6B1

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Tyr705 of human TrkB

ConjugateUnconjugated

Other NamesOBHD; TRKB; trk-B; EIEE58; GP145-TrkB

Accession NoSwiss-Prot:Q16620
GeneID:4915

Uniprot Q16620

Gene ID 4915

Calculated MWPredicted band size: 92 kDa

Sdspage MWObserved band size: 140 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : TrkB (Phospho-Tyr705) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with Lambda Protein Phosphatase for 30min whole cell
Lane 3 : 3T3 whole cell lysates
Lane 4 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human brain tissue stained for TrkB (Phospho-Tyr705) using 52705 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence TrkB (Phospho-Tyr705) antibody (52705)

ICC/IF staining of TrkB (Phospho-Tyr705) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 52705 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

If you have published an article using product 52705, please notify us so that we can cite your literature.

et al,TrkB-mediated neuroprotection in female hippocampal neurons is autonomous, estrogen receptor alpha-dependent, and eliminated by testosterone: a proposed model for sex differences in neonatal hippocampal neuronal injury. In Biol Sex Differ on 2024 Apr 2 by Vishal Chanana, Dila Zafer, et al..PMID:38566248 , (2024),
PMID: 38566248
et al,Vishal Chanana1, 2, Dila Zafer1, 2, Douglas B Kintner1, 2, Jayadevi H Chandrashekhar1, 3, Jens Eickhoff4 , (2024),
PMID:
et al,Pharmacological Co-Activation of TrkB and TrkC Receptor Signaling Ameliorates Striatal Neuropathology and Motor Deficits in Mouse Models of Huntington's Disease. , (2023),
PMID: 37638447

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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