Product Detail
Product NameTOP2A Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman
Immunogen DescA synthetic peptide of human TOP2A
ConjugateUnconjugated
Other NamesTOP2A;TOP2;TP2A
Accession NoSwiss Prot:P11388
Gene ID:7153
Uniprot
P11388
Gene ID
7153
Calculated MW174kDa/177kDa/178kDa/182kDa
Sdspage MW200kDa
FormulationBuffer: PBS with 0.02% sodium azide,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using TOP2A antibody.
Immunofluorescence analysis of U-2 OS cells using TOP2A Rabbit pAb.
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
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