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NF-kB p65 Rabbit Polyclonal Antibody#53227

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Product Detail

Product NameNF-kB p65 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human NF-kB p65 (NP_068810.3).

ConjugateUnconjugated

Other NamesNFKB3;p65;NF-kB p65;RELA;CMCU

Accession NoSwiss Prot:Q04206
Gene ID:5970

Uniprot Q04206

Gene ID 5970

Calculated MW58kDa/59kDa/60kDa

Sdspage MW65KDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody.
Immunohistochemistry of paraffin-embedded human colon using NF-kB p65 Rabbit pAb.
Immunohistochemistry of paraffin-embedded mouse lung using NF-kB p65 Rabbit pAb.
Immunohistochemistry of paraffin-embedded rat heart using NF-kB p65 Rabbit pAb.
Immunofluorescence analysis of C6 cells using NF-kB p65 Rabbit pAb.
Immunofluorescence analysis of NIH-3T3 cells using NF-kB p65 Rabbit pAb.
Immunofluorescence analysis of U-2 OS cells using NF-kB p65 Rabbit pAb.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

If you have published an article using product 53227, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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